Journal: bioRxiv

Article Title: A selective top-down pathway from anterior cingulate cortex embeds a representation of saliency in hippocampus

doi: 10.1101/2025.06.26.661852

Figure Lengend Snippet: (A) Dual retrograde labeling of ACC neurons. Left: The injection sites of retrograde AAVs in RSCd, RSCg and dmSTR. Right: The distribution of fluorescently tagged neurons in the ACC. (B) The proportion of dmSTR-projecting, RSCd-projecting, RSCg-projecting ACC neurons located in ACC layer 2/3 or layer 5. (C) The fraction of ACC projection neurons which project to each area. (D) Example images of sections of ACC labeled with retrograde AAV injected in RSCd/g and immunostained with anti-CaMKIIα or anti-GAD67 antibodies. (E) Similar to (D) but injections of retrograde AAV were targeted to dmSTR. (F) The proportion of RSCd/g-projecting or dmSTR-projecting ACC neurons which were labeled with anti-CaMKIIα or anti-GAD67.

Article Snippet: Prior to staining, slices were blocked in blocking buffer (0.1 M Tris-HCl, 0.15 M NaCl, 0.5% (w/v) blocking reagent (41116100, Roche)) at room temperature (RT) for 1 h. Slices were then washed with TNB-T (0.1 M Tris-HCl (pH 7.5), 0.15 M NaCl, 0.5% (w/v) blocking reagent, 1% Triton (R) X-100) twice for 2 min each and incubated with a monoclonal mouse anti-CaMKIIα antibody (1:250; 6G9, Abcam), or a polyclonal rabbit anti-GAD67 antibody (1:300; GTX 101881), or a monoclonal rabbit anti-c-Fos antibody (1:1,000; 9F6 #2250, Cell Signaling) in TNB-T overnight at 4 °C.

Techniques: Labeling, Injection